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Pangenome graph constructing from genome alignments with Minigraph-Cactus

Pangenome graph constructing from genome alignments with Minigraph-Cactus

Posted on May 11, 2023 By Tom Butwin No Comments on Pangenome graph constructing from genome alignments with Minigraph-Cactus
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Science nature

Recordsdata availability

Code availability

All source code for the Minigraph-Cactus pangenome pipeline, as neatly as free up binaries, Docker photos and particular person manuals, might perchance likely likely merely furthermore be stumbled on at https://github.com/ComparativeGenomicsToolkit/cactus.

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Download references

Acknowledgements

We thank A. D. Long for masses of solutions and insights in terms of the D. melanogaster recordsdata and the total vg team for his or her work to invent and dangle vg, upon which mighty of this work is dependent. B.P., A.N., J.M.E. and J.M. were partly supported by Nationwide Institutes of Health (NIH) grants R01HG010485, U24HG010262, U24HG011853, OT3HL142481, U01HG010961 (with H.L.) and OT2OD033761. H.L. became partly supported by NIH grant R01HG010040 and T.M. by U01HG010973. Computational infrastructure and toughen for working PanGenie were provided by the Centre for Recordsdata and Media Technology at Heinrich Heine University Düsseldorf.

Author recordsdata

Author notes

  1. These authors contributed equally: Glenn Hickey, Jean Monlong.

Authors and Affiliations

  1. UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA

    Glenn Hickey, Jean Monlong, Adam M. Novak, Jordan M. Eizenga, Mobin Asri, Xian H. Chang, Label Diekhans, Marina Haukness, David Haussler, Julian K. Lucas, Charles Markello, Karen H. Miga, Hugh E. Olsen, Trevor Pesout, Jouni Sirén & Benedict Paten

  2. Institute for Clinical Biometry and Bioinformatics, Clinical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany

    Jana Ebler & Tobias Marschall

  3. Middle for Digital Remedy, Heinrich Heine University Düsseldorf, Düsseldorf, Germany

    Jana Ebler, Daniel Doerr, Peter Ebert, Jana Ebler, Hugo Magalhães, Pierre Marijon, Tobias Marschall & Tobias Marschall

  4. Middle for Computational and Genomic Remedy, The Younger other folks’s Health heart of Philadelphia, Philadelphia, PA, USA

    Yan Gao

  5. Department of Recordsdata Sciences, Dana-Farber Most cancers Institute, Boston, MA, USA

    Haoyu Cheng, Justin Chu, Xiaowen Feng & Heng Li

  6. Department of Biomedical Informatics, Harvard Clinical College, Boston, MA, USA

    Haoyu Cheng, Xiaowen Feng, Carlos Garcia Giron & Heng Li

  7. Division of Oncology, Department of Internal Remedy, Washington University College of Remedy, St. Louis, MO, USA

    Haley J. Abel

  8. McDonnell Genome Institute, Washington University College of Remedy, St. Louis, MO, USA

    Lucinda L. Antonacci-Fulton, Sarah Cody, Robert S. Fulton, Allison A. Regier, Chad Tomlinson & Ting Wang

  9. Google LLC, Mountain Notice, CA, USA

    Gunjan Baid, Anastasiya Belyaeva, Andrew Carroll, Pi-Chuan Chang, Daniel E. Cook dinner, Alexey Kolesnikov, Maria Nattestad & Kishwar Shafin

  10. Department of Genome Sciences, University of Washington College of Remedy, Seattle, WA, USA

    Carl A. Baker, Evan E. Eichler, William T. Harvey, Kendra Hoekzema, Jennifer Kordosky, Alexandra P. Lewis, Katherine M. Munson, David Porubsky & Mitchell R. Vollger

  11. European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK

    Konstantinos Billis, Susan Fairley, Paul Flicek, Adam Frankish, Leanne Haggerty, Thibaut Hourlier, Jan O. Korbel, Fergal J. Martin & Francesca Floriana Tricomi

  12. Department of Human Genetics, McGill University, Montreal, QC, Canada

    Guillaume Bourque

  13. Canadian Middle for Computational Genomics, McGill University, Montreal, QC, Canada

    Guillaume Bourque

  14. Institute for the Superior Peep of Human Biology (WPI-ASHBi), Kyoto University, Kyoto, Japan

    Guillaume Bourque

  15. Institute of Genetics and Biophysics, Nationwide Research Council, Naples, Italy

    Silvia Buonaiuto & Vincenza Colonna

  16. Department of Quantitative and Computational Biology, University of Southern California, Los Angeles, CA, USA

    Label J. P. Chaisson & Tsung-Yu Lu

  17. Department of Genetics, Genomics and Informatics, University of Tennessee Health Science Middle, Memphis, TN, USA

    Vincenza Colonna, Christian Fischer, Erik Garrison, Andrea Guarracino, Pjotr Prins & Flavia Villani

  18. Arizona Instruct University, Barrett and O’Connor Washington Middle, Washington, DC, USA

    Robert M. Cook dinner-Deegan

  19. Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, Santa Cruz, CA, USA

    Omar E. Cornejo & Samuel Sacco

  20. Institute for Clinical Biometry and Bioinformatics, Clinical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany

    Daniel Doerr, Peter Ebert, Jana Ebler, Hugo Magalhães, Pierre Marijon & Tobias Marschall

  21. Core Unit Bioinformatics, Clinical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany

    Peter Ebert

  22. Howard Hughes Clinical Institute, Chevy Mosey, MD, USA

    Evan E. Eichler, David Haussler & Erich D. Jarvis

  23. Vertebrate Genome Laboratory, The Rockefeller University, New York, NY, USA

    Olivier Fedrigo, Giulio Formenti, Erich D. Jarvis & Jacquelyn Mountcastle

  24. Nationwide Institutes of Health (NIH)–Nationwide Human Genome Research Institute, Bethesda, MD, USA

    Adam L. Felsenfeld, Baergen I. Schultz, Michael W. Smith & Heidi J. Sofia

  25. Department of Genetics, Washington University College of Remedy, St. Louis, MO, USA

    Robert S. Fulton & Ting Wang

  26. Novo Nordisk Foundation Middle for Biosustainability, Technical University of Denmark, Copenhagen, Denmark

    Shilpa Garg

  27. Institute for Society and Genetics, College of Letters and Science, University of California, Los Angeles, Los Angeles, CA, USA

    Nanibaa’ A. Garrison

  28. Institute for Precision Health, David Geffen College of Remedy, University of California, Los Angeles, Los Angeles, CA, USA

    Nanibaa’ A. Garrison

  29. Division of Traditional Internal Remedy and Health Products and services Research, David Geffen College of Remedy, University of California, Los Angeles, Los Angeles, CA, USA

    Nanibaa’ A. Garrison

  30. Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA

    Richard E. Inexperienced

  31. Dovetail Genomics, Scotts Valley, CA, USA

    Richard E. Inexperienced

  32. Quantitative Life Sciences, McGill University, Montreal, QC, Canada

    Cristian Groza

  33. Genomics Research Centre, Human Technopole, Milan, Italy

    Andrea Guarracino

  34. Department of Genetics, Yale University College of Remedy, New Haven, CT, USA

    Ira M. Hall, Wen-Wei Liao & Shuangjia Lu

  35. Middle for Genomic Health, Yale University College of Remedy, New Haven, CT, USA

    Ira M. Hall & Wen-Wei Liao

  36. Quantitative Biology Middle (QBiC), University of Tübingen, Tübingen, Germany

    Simon Heumos

  37. Biomedical Recordsdata Science, Department of Computer Science, University of Tübingen, Tübingen, Germany

    Simon Heumos

  38. Tree of Life, Wellcome Sanger Institute, Hinxton, Cambridge, UK

    Kerstin Howe & Jonathan M. D. Wood

  39. Northeastern University, Boston, MA, USA

    Miten Jain

  40. Laboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA

    Erich D. Jarvis

  41. Division of Oncology, Department of Remedy, Stanford University College of Remedy, Stanford, CA, USA

    Hanlee P. Ji & HoJoon Lee

  42. Institute for Genomic Health, Icahn College of Remedy at Mount Sinai, New York, NY, USA

    Eimear E. Kenny

  43. Program in Bioethics and Institute for Human Genetics, University of California, San Francisco, San Francisco, CA, USA

    Barbara A. Koenig

  44. European Molecular Biology Laboratory, Genome Biology Unit, Heidelberg, Germany

    Jan O. Korbel

  45. Genome Informatics Half, Computational and Statistical Genomics Branch, Nationwide Human Genome Research Institute, Nationwide Institutes of Health, Bethesda, MD, USA

    Sergey Koren, Ann McCartney, Sergey Nurk, Adam M. Phillippy, Mikko Rautiainen, Arang Rhie & Brian Walenz

  46. Division of Biology and Biomedical Sciences, Washington University College of Remedy, St. Louis, MO, USA

    Wen-Wei Liao

  47. Computer Sciences Department, Barcelona Supercomputing Middle, Barcelona, Spain

    Santiago Marco-Sola

  48. Departament d’Arquitectura de Computadors i Sistemes Operatius, Universitat Autònoma de Barcelona, Barcelona, Spain

    Santiago Marco-Sola

  49. Self-discipline subject Measurement Laboratory, Nationwide Institute of Standards and Technology, Gaithersburg, MD, USA

    Jennifer McDaniel, Nathan D. Olson, Justin Wagner & Justin M. Zook

  50. Coriell Institute for Clinical Research, Camden, NJ, USA

    Matthew W. Mitchell

  51. Department of Computer Science, University of Pisa, Pisa, Italy

    Moses Njagi Mwaniki

  52. Department of Public Health Sciences, University of California, Davis, Davis, CA, USA

    Alice B. Popejoy

  53. Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA

    Daniela Puiu & Aleksey V. Zimin

  54. Berlin Institute for Clinical Systems Biology, Max Delbrück Middle for Molecular Remedy in the Helmholtz Association, Berlin, Germany

    Ashley D. Sanders

  55. Nationwide Middle for Biotechnology Recordsdata, Nationwide Library of Remedy, Nationwide Institutes of Health, Bethesda, MD, USA

    Valerie A. Schneider & Françoise Thibaud-Nissen

  56. Middle for Health Recordsdata Science, University of Copenhagen, Copenhagen, Denmark

    Jonas A. Sibbesen

  57. Al Jalila Genomics Middle of Excellence, Al Jalila Younger other folks’s Strong level Health heart, Dubai, UAE

    Ahmad N. Abou Tayoun

  58. Middle for Genomic Discovery, Mohammed Bin Rashid University of Remedy and Health Sciences, Dubai, UAE

    Ahmad N. Abou Tayoun

  59. Division of Clinical Genetics, University of Washington College of Remedy, Seattle, WA, USA

    Mitchell R. Vollger

  60. Middle for Computational Biology, Johns Hopkins University, Baltimore, MD, USA

    Aleksey V. Zimin

Consortia

Human Pangenome Reference Consortium

  • Haley J. Abel
  • , Lucinda L. Antonacci-Fulton
  • , Mobin Asri
  • , Gunjan Baid
  • , Carl A. Baker
  • , Anastasiya Belyaeva
  • , Konstantinos Billis
  • , Guillaume Bourque
  • , Silvia Buonaiuto
  • , Andrew Carroll
  • , Label J. P. Chaisson
  • , Pi-Chuan Chang
  • , Xian H. Chang
  • , Haoyu Cheng
  • , Justin Chu
  • , Sarah Cody
  • , Vincenza Colonna
  • , Daniel E. Cook dinner
  • , Robert M. Cook dinner-Deegan
  • , Omar E. Cornejo
  • , Label Diekhans
  • , Daniel Doerr
  • , Peter Ebert
  • , Jana Ebler
  • , Evan E. Eichler
  • , Jordan M. Eizenga
  • , Susan Fairley
  • , Olivier Fedrigo
  • , Adam L. Felsenfeld
  • , Xiaowen Feng
  • , Christian Fischer
  • , Paul Flicek
  • , Giulio Formenti
  • , Adam Frankish
  • , Robert S. Fulton
  • , Yan Gao
  • , Shilpa Garg
  • , Erik Garrison
  • , Nanibaa’ A. Garrison
  • , Carlos Garcia Giron
  • , Richard E. Inexperienced
  • , Cristian Groza
  • , Andrea Guarracino
  • , Leanne Haggerty
  • , Ira M. Hall
  • , William T. Harvey
  • , Marina Haukness
  • , David Haussler
  • , Simon Heumos
  • , Glenn Hickey
  • , Kendra Hoekzema
  • , Thibaut Hourlier
  • , Kerstin Howe
  • , Miten Jain
  • , Erich D. Jarvis
  • , Hanlee P. Ji
  • , Eimear E. Kenny
  • , Barbara A. Koenig
  • , Alexey Kolesnikov
  • , Jan O. Korbel
  • , Jennifer Kordosky
  • , Sergey Koren
  • , HoJoon Lee
  • , Alexandra P. Lewis
  • , Heng Li
  • , Wen-Wei Liao
  • , Shuangjia Lu
  • , Tsung-Yu Lu
  • , Julian K. Lucas
  • , Hugo Magalhães
  • , Santiago Marco-Sola
  • , Pierre Marijon
  • , Charles Markello
  • , Tobias Marschall
  • , Fergal J. Martin
  • , Ann McCartney
  • , Jennifer McDaniel
  • , Karen H. Miga
  • , Matthew W. Mitchell
  • , Jean Monlong
  • , Jacquelyn Mountcastle
  • , Katherine M. Munson
  • , Moses Njagi Mwaniki
  • , Maria Nattestad
  • , Adam M. Novak
  • , Sergey Nurk
  • , Hugh E. Olsen
  • , Nathan D. Olson
  • , Benedict Paten
  • , Trevor Pesout
  • , Adam M. Phillippy
  • , Alice B. Popejoy
  • , David Porubsky
  • , Pjotr Prins
  • , Daniela Puiu
  • , Mikko Rautiainen
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  • , Arang Rhie
  • , Samuel Sacco
  • , Ashley D. Sanders
  • , Valerie A. Schneider
  • , Baergen I. Schultz
  • , Kishwar Shafin
  • , Jonas A. Sibbesen
  • , Jouni Sirén
  • , Michael W. Smith
  • , Heidi J. Sofia
  • , Ahmad N. Abou Tayoun
  • , Françoise Thibaud-Nissen
  • , Chad Tomlinson
  • , Francesca Floriana Tricomi
  • , Flavia Villani
  • , Mitchell R. Vollger
  • , Justin Wagner
  • , Brian Walenz
  • , Ting Wang
  • , Jonathan M. D. Wood
  • , Aleksey V. Zimin
  •  & Justin M. Zook

Contributions

G.H., J.M., H.L. and B.P. designed the diagram. G.H., J.M. and J.E. contributed to the outcomes and diagnosis. G.H., J.M., A.N., J.E. and B.P. wrote the mansuscript. All authors contributed to the instrument. B.P. led the venture.

Corresponding authors

Correspondence to
Glenn Hickey or Benedict Paten.

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Hickey, G., Monlong, J., Ebler, J. et al. Pangenome graph constructing from genome alignments with Minigraph-Cactus.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01793-w

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  • Got: 06 October 2022

  • Licensed: 18 April 2023

  • Printed: 10 Would per chance 2023

  • DOI: https://doi.org/10.1038/s41587-023-01793-w

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Science and Nature Tags:graph, Pangenome, science

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